TY - JOUR AU - Haryani , Yuli AU - Melanie, Yonatha AU - Novita, Maria AU - Yuharmen, AU - Hendra, Rudi AU - Kartika , Ganis Fia PY - 2022/03/31 Y2 - 2024/03/28 TI - IAI SPECIAL EDITION: Green synthesis of silver nanoparticles from Alpinia galanga extract with microwave irradiation and antibacterial activity against Escherichia coli JF - Pharmacy Education JA - Pharm Educ VL - 22 IS - 2 SE - Special Edition DO - 10.46542/pe.2022.222.2023 UR - https://pharmacyeducation.fip.org/pharmacyeducation/article/view/1584 SP - p. 20-23 AB - <p><strong>Background: </strong>This study deals with the rapid green synthesis of silver nanoparticles (Ag NPs) by microwave irradiation using water extract of <em>Alpinia galanga</em> as a reducing agent. <strong>     </strong></p><p><strong>Methods:</strong> The effect of concentrations of water extract and silver nitrate aqueous solution (1 mM) on the synthesis was investigated using the ratios 1:1, 1:5, 1:10, and 1:20. The formation of Ag NPs was observed after being microwaved for 30, 60, 90, 120, 150, and 180 seconds.    </p><p><strong>Results:</strong> The best Ag NPs were produced from the ratio of 1:10 after 180 s irradiation, confirmed by the optimum absorbance at 450 nm using a UV-visible spectrophotometer. Transmission electron microscopy analysis showed that the size of the Ag NPs was 50 nm. Their antibacterial activity was determined using Resazurin microtiter assay against <em>Escherichia coli</em>, and the results showed that it inhibited 94.4% of <em>E. coli</em>. It was significantly different (<em>p</em> &lt; 0.05) with the inhibition of extract and Amoxsan, which inhibited 71.1% and 85.8% of <em>E. coli</em>, respectively.      </p><p><strong>Conclusion:</strong> The use of microwave irradiation on the synthesis of silver nanoparticles using <em>Alpinia galanga</em> extract as a reducing agent appears to be rapid and eco-friendly.</p> ER -