IAI CONFERENCE: In vitro antimalarial activity assay of Ashitaba Leaf ethanolic extract (Angelica keiskei)

Authors

  • Alvi Kusuma Wardani University of Muhammadiyah Mataram, Indonesia https://orcid.org/0000-0002-3042-6040
  • Abdul Rahman Wahid University of Muhammadiyah Mataram, Indonesia
  • Miftahul Jannah University of Muhammadiyah Mataram, Indonesia

DOI:

https://doi.org/10.46542/pe.2021.212.2730

Keywords:

Antimalarial, Ashitaba, Leaf, Plasmodium falciparum

Abstract

Introduction: The incidence of malaria is still very high in number in the world. Difficulty in treating malaria is caused by the resistance of malaria parasites to conventional drugs. An alternative treatment that can be used to treat malaria is to discover new drugs from natural ingredients.    

Aim: This study aimed to determine the activity of the Ashitaba leaf ethanolic extract as an antimalarial drug to Plasmodium falciparum strain 3D7.     

Methods: This study tested the activity of Ashitaba extract on the growth of P.falciparum in five concentrations, namely concentration of 0.01 ppm, 0.1 ppm, 1 ppm, 10 ppm, and 100 ppm.     

Results: The test results showed that the highest inhibitory effect was found on the concentration of 100 ppm with percent inhibition of 79.47 ± 26.91%. The 50% inhibition to parasites showed the half maximal inhibitory concentration (IC50) value of 2.09 ppm, compared to the positive control of which the IC50 of chloroquine was 0.007 ppm.    

Conclusion: Ashitaba leaf extract can be considered to have very active anti-malarial activity, because it has an IC50 value of less than 5 ppm.

Author Biographies

Alvi Kusuma Wardani, University of Muhammadiyah Mataram, Indonesia

Faculty of Health Sciences

Abdul Rahman Wahid, University of Muhammadiyah Mataram, Indonesia

Faculty of Health Sciences

Miftahul Jannah, University of Muhammadiyah Mataram, Indonesia

Faculty of Health Sciences

References

Achmadi.U.F. (2010). Manajemen Penyakit Berbasis Wilayah. Jakarta: Penerbit UI

Amalia.R. (2017). Formulasi dan Uji Aktivitas Antioksidan Sediaan Krim Ekstral Etanol 70% Daun Ashitaba (Angelica keiskei) dengan Setil Alkohol Sebagai Stiffening Anget. Jakarta UIN Syarif Hidayatullah

Departemen Kesehatan RI. (2006). Gebrak malaria. Pedoman pelaksanaan kasus malaria di Indonesia. Direktorat Jenderal Pengendalian Penyakit dan Penyehatan Lingkungan. Jakarta

Departemen Kesehatan RI. (2008). Pelayanan Kefarmasian Untuk Penyakit Malaria. Jakarta: Depkes RI

Dinas Kesehatan Provinsi NTB. (2018). Profil Kesehatan Provinsi NTB 2018. Dinas Kesehatan Provinsi NTB. Mataram

Djarwis.D. (2004). Teknik Penelitian Kimia Organik Bahan Alam, Workshop Peningkatan Sumber Daya Manusia. Penelitian Dan Pengelolahan Sumber Daya Hutan yang Berkelanjutan. Padang: Universitas Andalas

Ella.W. (2017). Uji Aktivitas Ekstrak Kasar Etanol dan Fraksi N-Heksan Tanaman Rumput Bamboo (Lophaterumgralie B.) sebagai antimalaria pada parasit Plasmodium Falciparum Strain 3D7. Skripsi. Ibrahim Malik. Jurusan Kimia FakultasSains dan Teknologi Universitas Islam Negeri Maulana Malik Ibrahim

Gasler. M.C., Nkunya. M.H.N., Mahasumbi. L. B., Heinrich. M., dan Toner. M. (1994). Screening Tanzanin Medical Plants for Antimalaria Activity. Journal Of Ethnopharmacology, 48(1): 131-144

Handayani, F.W., Muhtadi, A., Farmasi, F., Padjadjaran, U., Dara, T., Manis, K., & Aktif, S. (2013). Farmaka, 4, 1–15

Hans R.H, Guantai E.M, LateganC, Smith P.J, Wan B, Franzblau S.G, Gut, J., Roshenthal, PJ. & Chibale, K. (2010). Synthesis, Antimalarial, and Antitubercular Activity Of Acetylenic Chalcones. Bioorganic & medicinal chemistry letters, 20 (3): 924-4

Ilhami. F.Y., Fatma S. W., Elidahanum. H. (2013). Uji Efek Sitotoksik Hasil Fraksinasi Ekstrak Etanol Akar Asam Kandis (Garcinia Cowa Roxb) terhadap sel Kanker Payudara T47D dengan metode MTT. Laporan Penelitian. Fakultas Farmasi Universitas Andalas Padang

Khoplar SM. (2008). Konsep dasar kimia analitik. (Alih bahasa: A. Saptorahardjo). Jakarta: UI Press

Mustofa. (2009). Obat Antimalaria Baru: Antara Harapan dan Kenyataan. Pidato Pengukuhan Jabatan Guru Besar pada Fakultas Kedokteran. Yogyakarta

Sofi Fajriah & Megawati. (2015). Penapiasan Fitokimia dan Uji Toksisitas dan Daun Myristica FatuaHoutt. Depok: Departemen Kimia. FMIPA. UI

Sudoyo AW, Setyo hadi B, Alwi I. (2009). Buku Ajar Ilmu Penyakit Dalam. Jilid III Edisi V. Jakarta: Interna Publishing Pusat Penerbitan Ilmu Penyakit Dalam, 2773-2779

Wardani A.K, Wahid AR, Rosa NS. (2019). In Vitro antimalarial activity of Ashitaba Root Extracts (Angelica keiskei K.). Universitas Muhammadiyah Mataram. NTB

Wardani, A.K., Wahid, A.R., & Astuti, Y. (2020). Uji Aktivitas Antimalaria in vitro dari Ekstrak Etanol Batang Tanaman Ashitaba (Angelica keiskei [Miq.] Koidz ). 18(2), 202–206

World Health Organization. (2020). Malaria. Available at: https://www.who.int/news-room/fact-sheets/detail/malaria

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Published

28-07-2021

How to Cite

Wardani, A. K. ., Wahid, A. R. ., & Jannah, M. . (2021). IAI CONFERENCE: In vitro antimalarial activity assay of Ashitaba Leaf ethanolic extract (Angelica keiskei). Pharmacy Education, 21(2), p. 27–30. https://doi.org/10.46542/pe.2021.212.2730

Issue

Vol. 21 No. 2 (2021): IAI Conference 2020

Section

Conference Proceedings

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