IAI CONFERENCE: In vivo activity of Phaseolus vulgaris as an anti-hypercholesterolemic
DOI:
https://doi.org/10.46542/pe.2021.212.184188Keywords:
Antihyperlipidemic, Cholesterol, Extraction, Green beans, Phaseolus vulgarisAbstract
Introduction: Hyperlipidemia occurs due to increased levels of lipids and cholesterol in the blood. Phytosterols, such as stigmasterol in Phaseolus vulgaris, can reduce blood cholesterol levels.
Aims: The purpose of this study was to determine the activity of P. vulgarisin hypercholesterolemic rats.
Methods: Nine groups underwent the anti-hypercholesterolemia test: control group, negative group, positive group, 150 mg/kg body weight (bw) and 300 mg/kg bw n-hexane extract groups, 150 mg/kg bw and 300 mg/kg bw ethyl acetate extract groups, and 150 mg/kg bw and 300 mg/kg bw ethanol extract groups.
Results: All groups, except the control group, were given a high cholesterol diet to induce hypercholesterolemia (until the total cholesterol levels were higher than 200 mg/dL), followed by testing for ten days. The results showed that the 300 mg/kg bw ethyl acetate extract group had the best activity in reducing total cholesterol.
References
Agustina KK. (2015).Laboratory animal welfare. Anim Welf, 4–11
Artha C, Mustika A & Sulistyawati SW. (2017). Effect of Singawalang Leaf extract effects on LDL levels of hypercholesterolemic male rats. 5(2), 105–9
Chao-Feng Lin, Ya-Hui Chang, Shih-Chieh Chien, Yueh-Hung Lin & Hung-Yi Yeh. (2018). Epidemiology of dyslipidemia in the Asia Pacific Region. International Journal of Gerontology, 12(1), 2-6. https://doi.org/10.1016/j.ijge.2018.02.010
Fengel D & Wegener G. (1995). Kayu: kimia ultrastruktur reaksi-reaksi. Gadjah Mada University Press
Gazali M & Nufus H. (2019). Eksplorasi senyawa bioaktif ekstrak Daun Nipah (Nypa Fruticans Wurmb) asal pesisir Aceh Barat sebagai antioksidan. Jphpi, 22,155–63
Musdalifa N. (2017). Correlation between Body Mass Index and total cholesterol levels in staff and teachers in SMA Negeri 1 Kendari. e-journal UHO, 4(2), 361–7. https://doi.org/10.1109/TPWRD.2018.2825257
Mutia S. (2018). The effect of giving Andong leaf ethanol extract (Cordyline fruticosa (L.) A chev) on total cholesterol and blood triglyceride levels in rats (Rattus norvegicus) hypercholesterolemia. J Bioleuser, 2(2), 29–35
Naim F. (2017). Activity of Dutch Teak Leaf extract on HDL and LDL Cholesterol Levels in Hypercholesterolemic Rats. Life Sci, 6(1), 8
Ahmad, N. (2018). Extraction of natural compounds. Yogyakarta: Deepublish. 39–40
Normalisa. (2018). Effect of ethanol extract of beans (Phaseolus Vulgaris L.) on blood glucose levels and Malondialdehyde levels in diabetic rats. 53. https://doi.org/10.1017/CBO9781107415324.004
Onwe P, Folawiyo M, Ogah A, Umahi G, Okorocha A & Afoke A. (2015). Hyperlipidemia: etiology and possible control. IOSR J Dent Med Sci, 14(10), 2279–861. https://doi.org/10.9790/0853-1410693100
Pascale R, Bianco G, Cataldi TRI, Kopplin PS, Bosco F & Vignola L. (2018). Mass spectrometry-based phytochemical screening for hypoglycemic activity of Fagioli di Sarconi beans (Phaseolus vulgaris L.). Food Chem, 242, 497–504. https://doi.org/10.1016/j.foodchem.2017.09.091
Permadi A. (2018). Comparison of stratified and unstaged extraction methods of total flavonoids of Ciplukan Herbs by Colorimetry. Afif Permadi Sutanto Sri Wardatun, 19, 7
Putri, W.S, Warditiani, N.K, Larasanty LP. (2013). Phytochemical screening of ethyl acetate extract of mangosteen rind (Garcinia Mangostana L). 5
Rachmawani NR. (2017). The effect of beans (Phaseolus vulgaris L.) as alternative therapy Of Type 2 Diabetes Mellitus. Majority, 6(1), 71–6
Pascale R., Bianco G., Cataldi, TRI., Kopplin PS., Bosco F., Vignola L., Uhl J., Lucio M. & Milella L.. (2018). Mass spectrometry-based phytochemical screening for hypoglycemic activity of Fagioli di Sarconi beans (Phaseolus vulgaris L.). Food Chemistry, 242, 497-504. https://doi.org/10.1016/j.foodchem.2017.09.091
Ramadhani UP, Chandra B & Rifai H. (2020). Overview of phytochemistry and pharmacology of chickpeas (Phaseolus Vulgaris). World journal of pharmacy and pharmaceutical sciences, 9(9), 442-461
Rahayuningsih Nur & Tita Nofianti. (2015). The Antihyperlipidemia Effect of Strawberry Extract (Fragraria X Ananassa Duchesne). Jurnal Kesehatan Bakti Tunas Husada, 13(1), 213–27
Ratnayani K & Puspawati NR. (2016). Antioxidant and Activity Study Antibacteries on Enzimatic Hydraulic from Protein Kecambah Kacang Komak, Nut Gude And Beans. Proceeding Semnastect
Shattat G. F. (2014). A Review Article on Hyperlipidemia: Types, Treatments and New Drug Targets. Biomed Pharmacol J, 7(2)
Sri Wahjuni & Ni Luh Rustini P. (2016). Giving ethanol extract of Beans (Phaseolus Vulgaris L.) to lower total cholesterol, Low Density Lipoprotein (Ldl) And Increase High Density Lipoprotein (Hdl) In Wistar Rats on a High Fat Diet. 103–9
Tage, Novi. (2017). Standardization parameters of fresh plant Simplicia and ethanol extract of beans (Phaseolus vulgaris L.) from Three Different Regions. Univ Nusant PGRI Kediri, 1, 1–7. Available from: http://www.albayan.ae.
Wirawan W. (2018). Est of the effectiveness of Bay Leaf Fraction on total cholesterol levels of hypercholesterolemia-Diabetes male white rats. J Mandala Pharmacon Indones, 4(1), 74–82. https://doi.org/10.35311/jmpi.v4i1.27
