IAI CONFERENCE: Acute toxicity test of 96% ethanol extract of Syzygium myrtifolium leaves in white mice (Mus musculus)
DOI:
https://doi.org/10.46542/pe.2021.212.201204Keywords:
Acute toxicity test, Lethal dose 50% (LD50), Syzygium myrtifolium leavesAbstract
Introduction: Acute toxicity effects appear within a short time following the oral administration of either a single dose or repeated doses of toxin within 24 hours. Acute toxicity testing involves the administration of a range of doses across several groups of experimental animals with one dose administered per group, followed by the observation of toxic effects and mortality.
Aims: The purpose of this study was to determine the lethal dose 50 (LD50) and acute toxicity of an ethanol extract of Syzygium myrtifolium leaves in white mice.
Methods: Exposed groups consisted of a negative control group (carboxymethylcellulose sodium) and four treatment groups (500, 1000, 2000, and 4000 mg/kg body weight (bw)). Mortality was observed for 14 days following oral administration.
Results: The results demonstrated an LD50 of 1995 mg/kg bw, categorised as moderately toxic. Observed toxic effects included white lesions in the lungs, blackened liver, organ swelling, and fluid accumulation in the abdominal cavity and thorax.
References
Aisha, A.F.A., Ismail, Z., Abu-Salah, K.M., Shiddiqui, J.M., Gafar, G., & Majid, A.M.S.A. (2013). Syzygium myrtifolium Walp. Methanolic Extractinhiits Angiogenesis and Tumor Growth in Nude Mice. BMC Complementary and Alternative Medicine. 13, Article 168. https://doi.org/10.1186/1472-6882-13-168
Banks, W.J. (1993). Applied Veterinary Histology. Mosby. Texas
Departemen Kesehatan RI. (2014). Pedoman Uji Toksisitas Nonklinik Secara Invivo. Jakarta: Direktorat Jenderal Pengawasan Obat dan Makanan
Haryati, N.A., Saleh, C., & Erwin. (2015). Uji Toksisitas dan Aktivitas Antibakteri Ekstrak Daun Merah Tanaman Pucuk Merah (Syzygium myrtifolium Walp.) terhadap Bakteri Staphylococcus Aureus dan Escherichia Coli. Jurnal Kimia Mulawarman. 13 (1)
Indriani L, Almasyhuri, & Pratama AR. (2020). Aktivitas Gel Ekstrak Etanol Daun Pucuk Merah terhadap Penyembuhan Luka Bakar Tikus Sprague-dawley. Universitas Pakuan: Fitofarmaka: Jurnal Ilmiah Farmasi. https://doi.org/10.33751/jf.v10i2.2233
Katzung, B.G. (2002). Farmakologi dasar dan klinik. Jakarta: Salemba Medika
Kumar V, Cotran RS, & Robbins SL. (2007). Buku Ajar Patologi. Edisi 7; Alih Bahasa, Brahm U, Pendt; editor Bahasa Indonesia, Huriawati Hartanto, Nurwany Darmaniah, Nanda Wulandari. Ed-7. Jakarta: EGC.
Liniawati, S.R. Saleh, C., & Erwin. (2019). Isolasi dan Identifikasi Senyawa Triterpenoid dari Ekstrak n-Heksan Fraksi 8 Noda ke-2 dari Daun Merah Pucuk Merah (Syzygium myrtifolium Walp.). Jurnal Kimia Mulawarman, 16 (2), 73-77, May 2019
Montgomery, D.C. (1991). Design and Analysis of Experiment, 3rd edition. New York: Jhon Wiley and Sons
Priyanto. (2010). Toksikologi Ed: 2. Depok: Leskonfi Lembaga Studi dan Konsultasi Farmakologi
Rita, W.S., Suirta, I.W., & Sabikin, A. (2008). Isolasi dan Identifikasi Senyawa yang Berpotensi sebagai Antitumor pada Daging Buah Pare (Momordica charantia L.). Bukit Jimbaran: Skripsi Jurusan Kimia FMIPA Universitas Udayana
Yunita, E. (2009). Pengaruh Ekstrak Daun Teklan Terhadap Mortilitas dan Perkembangan Larva Aedes aegypti. BIOMA. 11 (1), 1410
