IAI SPECIAL EDITION: Antioxidant activity assay of Agarwood leaf extract cream (Aquilaria malaccensis L.) using free radical scavenging method
DOI:
https://doi.org/10.46542/pe.2022.222.2429Keywords:
Antioxidant Activity, DPPH, Agarwood leaf (A. malaccensis L.), Extract, CreamAbstract
Background: Agarwood leaves (A. malaccensis L.) are the part of the plant that is rich in antioxidants and contain phytochemicals, namely: alkaloids, flavonoids, terpenoids, steroids, and saponins.
Objective: This research aimed to determine the antioxidant activity of cream-containing Agarwood leaf extract using the DPPH (1,1-diphenyl-2-picrylhydrazyl) free radical.
Method: The sample was extracted by 70% ethanol and formulated at concentrations of 1% weight/volume (w/v), 3% w/v, and 5% w/v. Physical characteristics and antioxidant activity were evaluated.
Results: The results showed that the agarwood leaf extract cream in all concentrations fulfilled all the requirements related to physical characteristics of good topical preparations, including organoleptic, homogeneity, pH, spreadability, and adhesion. The IC50 values of the agarwood leaf extract cream at the concentrations of 1% w/v, 3% w/v, and 5% w/v were 68.371, 62.602, and 57.756 ppm, respectively.
Conclusion: This research concluded that agarwood leaf extract cream has high antioxidant or free radical scavenging properties (50 ppm-100 ppm).
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