IAI SPECIAL EDITION: Lung histopathological profile of male albino Wistar rats exposed to tobacco smoke administered ethanolic extract of red spinach
DOI:
https://doi.org/10.46542/pe.2022.222.142146Keywords:
Antioxidant, Alternathera amoena Voss, Lung histopathology, Tobacco smokeAbstract
Background: Exposure to tobacco smoke, which contains free radicals, can cause oxidative stress in the lung. Oxidative stress can trigger lung inflammation due to dilation of the alveolar lumen, alveolar wall thickening, and inflammatory cellular infiltration. Red spinach (Alternanthera amoena Voss.) leaves have antioxidant activity in vitro, with IC50 = 4.32 μg/mL.
Objective: To assess the antioxidant activity of red spinach in male albino Wistar rats.
Methods: The rats were divided into five test groups of five rats each: the negative control (1% Na-CMC suspension), the positive control (vitamin C suspension), and three experimental groups receiving red spinach leaves at a dose of 200 mg/Kg BW, 400 mg/Kg BW, and 600 mg/Kg BW, respectively. All the rats were exposed to tobacco smoke for 14 days using three cigarettes in a smoking chamber. The right lung was taken for histopathology.
Results: The results showed that doses of 200 mg/Kg BW, 400 mg/Kg BW, and 600 mg/Kg BW could reduce the level of lung damage, i.e. the enlarged alveolar lumen, thickened alveolar wall, and inflammatory cellular infiltration.
Conclusion: The dose of 600 mg/Kg BW was effective in reducing the level of lung damage compared to the negative control.
References
Abdelmohsen, U.R., Szesny, M., Othman, E. M., Schirmeister, T., Grond, S., Stopper, H., & Hentschel, U. (2012). Antioxidant and anti-protease activities of diazepinomicin from the sponge-associated Micromonospora strain RV115. Marine drugs, 10(10), 2208–2221. https://doi.org/10.3390/md10102208
Baker, Henry. J, Lindsey, J. Russel, Weisbroth, Steven. H. (1979). The Labolatory Rat. New York: Academic Press
Bernard, D., Kwabena, A., Osei, O., Daniel, G., Elom, S., & Sandra, A. (2014). The Effect of Different Drying Methods on the Phytochemicals and Radical Scavenging Activity of Ceylon Cinnamon (Cinnamomum zeylanicum) Plant Parts. European Journal of Medicinal Plants, 4(11), 1324–1335. https://doi.org/10.9734/ejmp/2014/11990
Cazarolli, L., Zanatta, L., Alberton, E., Bonorino Figueiredo, M.S., Folador, P., Damazio, R., Pizzolatti, M.G., Barreto Silva, F.R. (2008). Flavonoids: Prospective Drug Candidates. Mini-Reviews in Medicinal Chemistry, 8(13), 1429–1440. https://doi.org/10.2174/138955708786369564
Chen, Z., Wang, D., Liu, X., Pei, W., Li, J., Cao, Y., Zhang, J., An, Y., Nie, J., Tong, J. (2015). Oxidative DNA damage is involved in cigarette smoke-induced lung injury in rats. Environmental Health and Preventive Medicine, 20(5), 318–324. https://doi.org/10.1007/s12199-015-0469-z
Fitria, Triandini, R.C., Mangimbulude, J., & Karwur, F.F. (2014). Smoking and DNA Oxidation. Sains Medika, 5(2), 121–127. https://doi.org/10.20473/ijph.v11i1.2016.78-88
Idrus, H.R.Al, Iswahyudi, I., & Wahdaningsih, S. (2016). Antioxidant Activity Test of Ethanol Extract of Leeks of Mecca (Eleutherine americana Merr.) Against Histopathological Picture of Wistar Males (Rattus norvegicus) Lung After Exposure to Cigarette Smoke. Jurnal Fitofarmaka Indonesia, 1(2). https://doi.org/10.33096/jffi.v1i2.190
Lago, J.H.G., Toledo-Arruda, A.C., Mernak, M., Barrosa, K.H., Martins, M.A., Tibério, I.F.L.C., & Prado, C.M. (2014). Structure-Activity association of flavonoids in lung diseases. Molecules, 19(3), 3570–3595. https://doi.org/10.3390/molecules19033570
Lopes, A., Moura, R.S. De, Resende, A., & Porto, L.C. (2011). Mate Tea Meliorates emphysema in Cigarette Smoke-Exposed Mice. Experimental lung research, 37(4), 246–257. https://doi.org/10.3109/01902148.2010.535092
Marzouk, M.M. (2016). Flavonoid constituents and cytotoxic activity of Erucaria hispanica (L.) Druce growing wild in Egypt. Arabian Journal Of Chemistry, 9, S411-S415. https://doi.org/10.1016/j.arabjc.2011.05.010
Muchtadi, Dedy. (2009). Pengantar Ilmu Gizi. Bandung : Alfabeta.
Munhoz, V.M., Longhini, R., Souza, J.R.P., Zequi, J.A.C., Mello, E.V.S.L., Lopes, G.C., & Mello, J.C.P. (2014). Extraction of flavonoids from Tagetes patula : Process optimisation and screening for biological activity Original article Extraction of flavonoids from Tagetes patula : process optimisation and screening for biological activity. Revista Brasileira de Farmacognosia, 24(5), 576–583. https://doi.org/10.1016/j.bjp.2014.10.001
Schaal, C., & Chellappan, S.P. (2014). Nicotine-mediated cell proliferation and tumor progression in smoking-related cancers. Molecular Cancer Research, 12(1), 14–23. https://doi.org/10.1158/1541-7786.MCR-13-0541
Tohomi, K.L., Iswahyudi, & Wahdaningsih, S. (2014). Antioxidant Activity Test of the Ethanol Extract of the Leaves of Buas-Buas (Premna cordifolia Linn.) Against Histopathological Appearance of the Lungs of Rats (Rattus Norvegicus) of Male Wistar After Exposure to Cigarette Smoke
Wang, Q., Jin, J., Dai, N., Han, N., Han, J., & Bao, B. (2016). Anti-inflammatory effects, nuclear magnetic resonance identification, and high-performance liquid chromatography isolation of the total flavonoids from Artemisia frigida. Journal of food and drug analysis, 24(2), 385–391. https://doi.org/10.1016/j.jfda.2015.11.004
Ye, X.J., & Ng, T.B. (2011). Antitumor and HIV-1 Reverse Transcriptase Inhibitory Activities of a Hemagglutinin and a Protease Inhibitor from Mini-Black Soybean. Evidence-based complementary and alternative medicine: eCAM, 2011, 851396. https://doi.org/10.1155/2011/851396
Zahara, N.E., & Tjiptaningrum, A. (2019). Effect of Thymoquinone on the Lungs of Sprague Dawley Rats (Rattus norvegicus) Exposed to Cigarette Smoke Based on Histopathological Features (Rattus norvegicus). 8, 78–83
Ziech, D., Franco, R., Pappa, A., & Panayiotidis, M.I. (2011). Reactive oxygen species (ROS)--induced genetic and epigenetic alterations in human carcinogenesis. Mutation research, 711(1-2), 167–173. https://doi.org/10.1016/j.mrfmmm.2011.02.015
