IAI SPECIAL EDITION: In vitro anti-ageing activity of ethanol extract of Cantigi (Vaccinium varingiaefolium Blume Miq.) leaf and the extract loaded gelatin nanoparticles
DOI:
https://doi.org/10.46542/pe.2022.222.151155Keywords:
Anti-ageing, Cantigi, Extract, In vitro, NanoparticlesAbstract
Background: Empirically, Cantigi leaves (Vaccinium varingiaefolium Blume Miq.) have been used for beauty and health, but their activity has not been studied much.
Objective: To produce an ethanolic extract of Cantigi leaves, nanoparticles of the ethanolic extract and to compare their antioxidant, anticollagenase, and antityrosinase activities.
Methods: Dry powders of young Cantigi leaves were macerated gradually with hexane, ethyl acetate, and ethanol. Dried extracts were evaluated for specific and non-specific parameters, made into nanoparticles by desolvation using gelatin as polymer and glutaraldehyde as a crosslinker, and then characterized. Antioxidant, anticollagenase, and antityrosinase activities of the extracts and nanoparticles (IC50) were measured and compared.
Results: The ethanol extract of Cantigi leaves met specific and non-specific parameters. Nanoparticle: particle size of 307.90+2.54 nm, polydispersity index of 0.271+0.01, zeta potential 12.80+0.44 mV, entrapment efficiency of 43.29±3.97%, and spherical. IC50 of anticollagenase, antioxidant, and antityrosinase for extracts 104.18±0.33, 18.87±0.36, 196.88±20.28 ppm; and for nanoparticles 124.40±0.39, 30.14±0.33, and 53.55±27.07 ppm, respectively.
Conclusion: In general, the nanoparticle activity was lower than the extract activity except for the antityrosinase activity. The slow release of the extract from the nanoparticles is thought to be the cause of the decrease in activity.
References
Desmiaty, Y., Saputri, F.C., Hanafi, M., Prastiwi, R., Elya, B. (2020). Anti-elastase, anti-tyrosinase, and antioxidant of Rubus fraxinifolius stem methanolic extract. Pharmacognosy Journal, 12(2), 271-275. https://doi.org/10.5530/pj.2020.12.42
Ditjen POM RI. (2000). Parameter Standar Umum Ekstrak Tumbuhan Obat. Cetakan Pertama. Depkes RI, Jakarta
Foox, M., Zilberman, M. (2015). Drug delivery from gelatin-based systems, Expert Opinion on Drug Delivery, 12(9), 1547-1563, https://doi.org/10.1517/17425247.2015.1037272
Fytianos, G., Rahdar, A., Kyzas, G.Z. (2020). Nanomaterials in cosmetics: Recent updates. Nanomaterials, 10, 979. https://doi.org/10.3390/nano10050979
Khan, S.A. (2014). Gelatin nanoparticles as potential nanocarriers for macromolecular drugs. Dissertation. Phillips University Marburg, Marburg
Kosasih, K., Yulyana, A., Winarno, H. (2016). Karakterisasi ekstrak daun Cantigi (Vaccinium varingiaefolium (Blume) Miq.). Jurnal Sains dan Kesehatan, 1(5), 276-283. https://doi.org/10.25026/jskv1i5.50
Kosasih, K., Sumaryono, W., Supriyono, A., Mudhakir, D., Cristian, Y.E., Debora, R. (2019). Effects of gelatin and glutaraldehyde concentrations on characteristics of cantigi (Vaccinium varingiaefolium Miq.) extract loaded gelatin nanoparticles as antioxidant. The 2019 International Joint Symposium, Universitas Airlangga, Surabaya
Maya I. (2020). Cantigi ungu, anti penuaan dini dan antioksidan alami (online). Available at: https://farmasetika.com/ 2020/ 01/29/cantigi-ungu-anti-penuaan-dini-dan antioksidan-alami/
Moon, J-Y., Yim, E-Y., Song, G., Lee, N.H., Hyun, C-G. (2010). Screening of elastase and tyrosinase inhibitory activity from Jeju Island plants. Eurasian Journal of Bioscience, 53(March), 41-53. https://doi.org/10.5053/ejobios.2010.4.0.6
Nurrochmad, A., Wirasti, Dirman, A., Lukitaningsih, E., Rahmawati, A., Fakhrudin, N. (2018). Effects of antioxidant, anticollagenase, antielastase, antityrosinase of the extract and fraction from Turbinaria decurrens Bory. Indonesian Journal of Pharmacy, 29(4), 188–197. https://doi.org/10.14499/indonesianj pharm29iss4pp188
Pires, T.C.S.P., Caleja, C., Santos-Buelga, C., Barros, L., Ferreira, I.C.F.R. (2020). Vaccinium myrtillus L. fuits as a novel source of phenolic compounds with health benefits and industrial applications - A review. Curren Pharmaceutical Design, 26(16), 1917-1928. https://doi.org/ 10.2174/1381612826666200317132507
Regulation (EC) No. 1223/2009 (online). Available at: https://ec.europa.eu/growth/sectors/ cosmetics/ products/ nanom Taterials_en (accessed on 8 August 2021)
Sholikhah, A., Dian, F.A., Listyorini, D. (2017). Anatomy and morphological study of Mentigi Gunung (Vaccinium varingiaefolium (Blume) Miq.) in area of Mount Batok, Indonesia. KnE Life Science, 36-45. https://doi.org/ 10. 18502/ kls.v3i4.685
Syukri, Y., Purwati, R., Hazami, N., Tahmid, H.A., Fitria, A. (2020). Standardization of specific and non-specific parameters of Propolis extract as raw material for herbal product. Eksakta, 1(1), 36-43. https://doi.org/10.20885/EKSAKTA.vol1.iss1.art6