Protective effects of Piper crocatum ethanol extract on Gentamicin-induced nephrotoxicity in rats
DOI:
https://doi.org/10.46542/pe.2023.234.3236Keywords:
Antioxidant, Gentamicin, Piper crocatum, NephroprotectiveAbstract
Background: The kidney is one of the main target organs of toxic substances, including drugs. Gentamicin-induced nephrotoxicity is thought to be caused by the formation of oxidative stress and can be prevented by antioxidants. Piper crocatum has potent antioxidant activity.
Objective: This study aims to determine the nephroprotective activity of Piper crocatum.
Method: The study used Wistar rats in five groups (normal, negative, and ethanol extract of Piper crocatum with doses of 250, 500, and 750 mg/kg BW). The nephrotoxicity was induced by gentamicin for eight days. On the ninth day, the kidney function parameters (blood, urea, nitrogen, creatinine and uric acid) and malondialdehyde levels were measured, and kidney histology analysis was performed.
Results: The results demonstrated that the extracts show lower kidney function parameters and malondialdehyde levels than the negative control.
Conclusion: Piper crocatum is a potential nephroprotective agent.
References
Aiswarya, N., Rashmi, R.R., Preethi, S.J., Chandran, V., Teerthanath, S., Sunil, P.B., Rakesh, K.B. (2018). Nephroprotective effect of aqueous extract of Pimpinella anisum in gentamicin induced nephrotoxicity in Wistar rats. Pharmacognosy Journal, 10(3):403–407 https://doi.org/10.5530/pj.2018.3.66
Ayala, A., Muñoz, M.F. & Argüelles, S. (2014). Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal. Oxidative Medicine and Cellular Longevity, 2014. https://doi.org/10.1155/2014/360438
Balakumar, P., Rohilla, A. & Thangathirupathi, A. (2010). Gentamicin-induced nephrotoxicity: Do we have a promising therapeutic approach to blunt it? Pharmacological Research, 62:179–186 https://doi.org/10.1016/j.phrs.2010.04.004
Chawla, L.S. & Kimmel, P.L. (2012). Acute kidney injury and chronic kidney disease: an integrated clinical syndrome. Kidney International, 82(5):516–524 https://doi.org/10.1038/ki.2012.208
Elgebaly, H., Mosa, N.M., Germoush, M. & Brahim, A.C. (2016). The Nephro-Protective Effects of Olive Oil and Bee Honey against Gentamicin-Induced Nephrotoxicity in Rabbits. Aljouf University Medical Journal, 3(2)
Fristiohady, A., Wahyuni, W., Ilyas, M., Bafadal, M., Purnama, L.O.M.J., Sangadji, F. & Sahidin, I. (2020). Nephroprotective effect of extract Etlingera elatior (Jack) R.M. Smith on CCl4-induced nephrotoxicity in rats. Current Research on Biosciences and Biotechnology, 1(2):62–65 https://doi.org/10.5614/crbb.2019.1.2/dapm6106
Gheith, I. & El-Mahmoudy, A. (2018). Novel and classical renal biomarkers as evidence for the nephroprotective effect of Carica papaya leaf extract. Bioscience Reports, 38(5):1–10 https://doi.org/10.1042/BSR20181187
Kurajoh, M., Fukumoto, S., Yoshida, S., Akari, S. & Murase, T. (2021). Uric acid was shown to contribute to increased oxidative stress levels independent of xanthine oxidoreductase activity in the MedCity21 health examination registry. Scientific Reports, 1–9 https://doi.org/10.1038/s41598-021-86962-0
Laksmitawati, D., Widyastuti, A., Karami, N., Afifah, E., Rihibiha, D.R., Nufus, H. & Widowati, W. (2017). Inflammatory effects of Anredera cordifolia and Piper crocatum extracts on the lipopolysaccharide-stimulated macrophage cell line. Bangladesh Journal of Pharmacology, 12:35–40 https://doi.org/10.3329/bjp.v12i1.28714
Lin, S., Zhang, G., Liao, Y., Pan, J. & Gong, D. (2015). Dietary Flavonoids as Xanthine Oxidase Inhibitors: Structure − Affinity and Structure − Activity Relationships. Journal of Agriculture and Food Chemistry, 63(35):7784–7794 https://doi.org/10.1021/acs.jafc.5b03386
Lister, I.N.E., Ginting, C.N., Girsang, E., Nataya, E.D., Azizah, A.M. & Widowati, W. (2020). Hepatoprotective properties of red betel (Piper crocatum Ruiz and Pav ) leaves extract towards H2O2 -induced HepG2 cells via anti-inflammatory, anti necrotic, antioxidant potency. Saudi Pharmaceutical Journal, 28(10):1182–1189 https://doi.org/10.1016/j.jsps.2020.08.007
Liu, N., Xu, H., Sun, Q., Yu, X., Chen, W., Wei, H. & Lu, W. (2021). The Role of Oxidative Stress in Hyperuricemia and Xanthine Oxidoreductase (XOR) Inhibitors. Oxidative Medicine and Cellular Longevity, 2021 https://doi.org/10.1155/2021/1470380
Mahmoudzadeh, L., Najafi, H., Ashtiyani, S.C. & Mohamadi, Z. (2017). Anti-inflammatory and protective effects of saffron extract in ischemia- reperfusion-induced acute kidney injury. Nephrology, 22(10):748–754 https://doi.org/10.1111/nep.12849
Mehan, S., Dudi, R. & Kaira, S. (2017). Renoprotective Effect of Corosolic Acid in Gentamicin- Induced Nephrotoxicity and Renal Dysfunction in Experimental rats. Annals of Pharmacology and Pharmaceutics, 2(6):1065
Morales-Alvarez, M.C. (2020). Nephrotoxicity of Antimicrobials and Antibiotics. Advances in Chronic Kidney Disease, 27(1):31–37 https://doi.org/10.1053/j.ackd.2019.08.001
Namuleme, C.B., Ikwap, K., Tamale, A., Tumwine, G., Kateregga, J. & Kato, C.D. (2017). Nephroprotective Effect of Phoenix Reclinata Total Crude Root Extract on Tenofovir Induced Kidney Damage in Wistar Albino Rats. Journal of Pharmaceutical Research International, 17(6):1–10 https://doi.org/10.9734/JPRI/2017/34247
Sharma, V., Kaushik, A., Dey, Y., Srivastava, B., Wanjari, M., Pawar, S. & Chougule, S. (2021). The nephroprotective potential of Anogeissus latifolia Roxb. (Dhava) against gentamicin-induced nephrotoxicity in rats. Journal of Ethnopharmacology, 273(2018):114001 https://doi.org/10.1016/j.jep.2021.114001
Suri, M., Azizah, Z. & Asra, R. (2021). Traditional Use, Phytochemical and Pharmacological Review of Red Betel Leaves (Piper crocatum Ruiz & Pav) Asian Journal of Pharmaceutical Research and Development, 9(1):159–163
Yarijani, Z.M., Najafi, H. & Madani, S.H. (2016). Protective effect of crocin on gentamicin-induced nephrotoxicity in rats. Iranian Journal of Basic Medical Sciences, 19:337–343
