Increased levels of IL-10 in the spleen after induction of pili protein 65.5 kDa Klebsiella pneumoniae
DOI:
https://doi.org/10.46542/pe.2023.234.304310Keywords:
IL-10, Klebsiella pneumoniae, Protein pili, SpleenAbstract
Background: One of the gram-negative bacteria often found as the cause of nosocomial infections is Klebsiella pneumoniae. This bacterium has a mortality rate of 28.3% due to ESBL strains that cause resistance to several antibiotics and the absence of a vaccine as a preventive measure.
Objective: To determine the level of IL-10 in the spleen after induction of the protein pili K. pneumoniae 65.5 kDa in mice.
Method: This study used mice spleen samples from 21 mice aged six to eight weeks in an experimental investigation with a randomised posttest-only control group design. There are three groups, K1 as a PBS-given control, 65.5 kDa antigen protein pills + Freund’s adjuvants are given to K2, and Freund's adjuvants are given to K3. Interleukin-10 concentrations were measured using the ELISA and analysed by a one-way ANOVA assay.
Result: The research showed significant differences in each group at IL-10 levels after administration of the pili protein K. pneumoniae using one-way ANOVA (p = 0.036). The treatment group had the highest average levels of IL-10, so there was an increased association between exposure to pili protein and IL-10 levels in rats. A significant increase in IL-10 levels was found in the treatment group compared to the control and adjuvant groups.
Conclusion: Induction of protein pili Klebsiella pneumoniae 65.5 kDa increases IL-10 levels in spleen mice.
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