Identification of Δ9-tetrahydrocannabinol compounds in Cannabis sativa using gas chromatography-mass spectrometry
DOI:
https://doi.org/10.46542/pe.2024.246.4349Keywords:
Cannabis sativa, Duquenois, GC-MS, Narcotic, Narcotic abuseAbstract
Background: Narcotics are divided into three types natural, semisynthetic and synthetic. Cannabis is included in the Cannabaceae family with the species Cannabis sativa L. This plant is known as a plant that has psychotropic effects because it contains the main alkaloids cannabinol, cannabidiol, and Δ9-Tetrahydrocannabinol.
Objective: This study aims to identify the presence of Δ9-Tetrahydrocannabinol in samples.
Method: The method used is the Duquenois colour test, Fast Blue, Gas Chromatography-Mass Spectrometry (GC-MS) confirmation.
Result: The result shows that Dequenoise test positive giving a blue color and Fast Blue color test positive giving a brick red. The GC-MS analysis shows the presence of Δ9-Tetrahidrocannabivarin, Cannabichrome, Dronabinol, Cannabigerol, and Cannabinol. The chromatogram revealed five peaks, with the main compound identified as dronabinol.
Conclusion: Five compounds found in sample were 9-tetrahydrocannabivarin, cannabichromene, dronabinol, cannabigerol, and cannabinol, which were positive for marijuana.
References
Acosta, A., & Almirall, J. (2021). Differentiation between hemp-type and marijuana-type cannabis using the Fast Blue BB colorimetric test. Forensic Chemistry, 26, 100376. https://doi.org/10.1016/j.forc.2021.100376
Alves, P., Amaral, C., Teixeira, N., & Correia-da-Silva, G. (2020). Cannabis sativa: Much more beyond Δ9-tetrahydrocannabinol. Pharmacological Research, 157, 104822. https://doi.org/10.1016/j.phrs.2020.104822
Badan Narkotika Nasional. (2022). BNN musnahkan 78,2 Kg sabu dan 61,9 Kg ganja. https://bnn.go.id/bnn-musnahkan-782-kg-sabu-619-kg-ganja/
Chandra, S., Lata, H., & ElSohly, M. A. (Eds.). (2017). Cannabis sativa L. - Botany and biotechnology. Springer International Publishing. https://doi.org/10.1007/978-3-319-54564-6
ElSohly, M. A., Radwan, M. M., Gul, W., Chandra, S., & Galal, A. (2017). Phytochemistry of Cannabis sativa L. In A. D. Kinghorn, H. Falk, S. Gibbons, & J. Kobayashi (Eds.), Phytocannabinoids: Unraveling the complex chemistry and pharmacology of Cannabis sativa (pp. 1–36). Springer International Publishing. https://doi.org/10.1007/978-3-319-45541-9_1
Hazekamp, A., Peltenburg, A., Verpoorte, R., & Giroud, C. (2005). Chromatographic and spectroscopic data of cannabinoids from Cannabis sativa L. Journal of Liquid Chromatography and Related Technologies, 28(15), 2361–2382. https://doi.org/10.1080/10826070500187558
Honour, J. W. (2006). Gas chromatography-mass spectrometry. In M. J. Wheeler & J. S. M. Hutchinson (Eds.), Hormone assays in biological fluids (pp. 53–74). Humana Press. https://doi.org/10.1385/1-59259-986-9:53
Isnaini, E. (2017). Penggunaan ganja dalam ilmu pengobatan menurut Undang-Undang Nomor 35 Tahun 2009 tentang narkotika. Jurnal Independent, 5(2), 46. https://doi.org/10.30736/ji.v5i2.71
Jacobs, A. D., & Steiner, R. R. (2014). Detection of the Duquenois-Levine chromophore in a marijuana sample. Forensic Science International, 239, 1–5. https://doi.org/10.1016/j.forsciint.2014.02.031
McPartland, J. M. (2018). Cannabis systematics at the levels of family, genus, and species. Cannabis and Cannabinoid Research, 3(1), 203–212. https://doi.org/10.1089/can.2018.0039
National Center for Biotechnology Information. (2023). PubChem compound summary for CID 16078, Dronabinol.
Pitri Susanti, N. (2012). Identifikasi kandungan cannabinoid dalam ekstrak batang ganja dengan metode Al-Tlc dan Hptlc spectrophotodensitometry. Indonesian Journal of Legal and Forensic Sciences, 2(1), 282193. https://ojs.unud.ac.id/index.php/ijlfs/article/view/3249
Puslitdatin BNN. (2022). Indonesia drugs report 2022. Pusat Penelitian, Data, Dan Informasi Badan Narkotika Nasional (PUSLITDATIN BNN) (p. 24).
Sparkman, O. D., Penton, Z. E., & Kitson, F. G. (2011). Gas chromatography and mass spectrometry: A practical guide (2nd ed.). Academic Press. https://doi.org/10.1016/B978-0-12-373628-4.00056-3
Thomas, B. F., & ElSohly, M. A. (2016). The botany of Cannabis sativa L. In The analytical chemistry of Cannabis (Issue December 2016). https://doi.org/10.1016/b978-0-12-804646-3.00001-1
Undang Undang RI. (2009). Undang Undang RI 2009 tentang Narkotika.
United Nations Office on Drug and Crime. (2022a). Recommended methods for the identification and analysis of cannabis and cannabis products. https://www.unodc.org/documents/scientific/Recommended_methods_for_the_Identification_and_Analysis_of_Cannabis_and_Cannabis_products.pdf
United Nations Office on Drug and Crime. (2022b). World drug report 2022. https://www.unodc.org/unodc/en/data-and-analysis/world-drug-report-2022.html
Watanabe, K., Honda, G., Miyagi, T., & Kanai, M. (2017). The Duquenois reaction revisited: Mass spectrometric estimation of chromophore structures derived from major phytocannabinoids. Forensic Toxicology, 35, 185–189. https://doi.org/10.1007/s11419-016-0337-6