In vitro and in silico activities of Solanum melongena and Physalis angulata in DPP-IV inhibition in Diabetes mellitus
DOI:
https://doi.org/10.46542/pe.2024.243.406411Keywords:
Diabetes mellitus, DPP-IV, Physalis angulata, Solanum melongenaAbstract
Background: Impaired insulin secretion causes type 2 diabetes mellitus. The DPP-IV affects the secretion of insulin. DPP-IV inhibition is a potential therapeutic approach because it ensures optimal insulin secretion. Solanaceae family plants have the potential to inhibit the DPP-IV because of their phytochemicals, such as flavonoids and phenolic.
Objective: This study aimed to evaluate the DPP-IV inhibitory activity of Solanum melongena and Physalis angulata.
Method: The fruits were crushed and extracted. Phytochemical screening and total flavonoids and phenol contents of the extracts were determined. The DPP-IV inhibition assay was done via molecular docking, and the in vitro study was conducted to measure the percentage of inhibition.
Result: S. melongena had a higher content of total flavonoids and phenolics. In the docking study, ferulic acid and withangulatin A showed the best binding activity with DPP-IV compared to other compounds. P. angulata had a higher DPP-IV inhibition percentage than S. melongena.
Conclusion: S. melongena and P. angulata had DPP-IV inhibitory activity.
References
Abo, K., & Lawal, I. (2013). Antidiabetic activity of Physalis angulata extracts and fractions in alloxan-induced diabetic rats. Journal of Advanced Scientific Research, 4(3), 32–36. https://www.sciensage.info/index.php/JASR/article/view/167
Chhabria, S., Mathur, S., Vadakan, S., Sahoo, D., Mishra, P., & Paital, B. (2022). A review on phytochemical and pharmacological facets of tropical ethnomedicinal plants as reformed DPP-IV inhibitors to regulate incretin activity. Frontiers in Endocrinology, 13, 1–24. https://doi.org/10.3389/fendo.2022.1027237
Deacon, C. (2019). Physiology and pharmacology of DPP-4 in glucose homeostasis and the treatment of type 2 diabetes. Frontiers in Endocrinology, 10, 1‒14. https://doi.org/10.3389/fendo.2019.00080
Egwim, E., Hamzah, R., & Erukainure, O. (2013). Hypoglycemic potency of selected medicinal plants in Nigeria. Croatian Journal of Food Technology, Biotechnology and Nutrition, 8(3–4), 111–114. https://hrcak.srce.hr/115927
Gallwitz, B. (2019). Clinical use of DPP-4 inhibitors. Frontiers in Endocrinology, 10, 1–10. https://doi.org/10.3389/fendo.2019.00389
Gao, Y., Zhu, J., Li, Z., Zhu, W., Shi, J., Jia, Q., & Li, Y. (2015). Recent progress in natural products such as DPP-4 inhibitors. Future Medicinal Chemistry, 7(8), 1079–1089. https://doi.org/10.4155/fmc.15.49
Huang, P., Lin, S., Chang, C., Tsai, M., Lee, D., & Weng, C. (2019). Natural phenolic compounds potentiate hypoglycemia via inhibition of dipeptidyl peptidase IV. Scientific Reports, 9(1), 1–11. https://doi.org/10.1038/s41598-019-52088-7
Ivanova, L., & Karelson, M. (2022). The impact of software used and the type of target protein on molecular docking accuracy. Molecules, 27(24). https://doi.org/10.3390/molecules27249041
Kandimalla, R., Kalita, S., Choudhury, B., & Kotoky, J. (2015). A review of the antidiabetic potential of the genus Solanum (Solanaceae). Journal of Drug Delivery and Therapeutics, 5(1), 24–27. https://doi.org/10.22270/jddt.v5i1.1026
Li, K., Yao, F., Xue, Q., Fan, H., Yang, L., Li, X., Sun, L., & Liu, Y. (2018). Inhibitory effects against α-glucosidase and α-amylase of the flavonoids-rich extract from Scutellaria baicalensis shoots and interpretation of the structure-activity relationship of its eight flavonoids by a refined assign-score method. Chemistry Central Journal, 12(1), 1–11. https://doi.org/10.1186/s13065-018-0445-y
Luo, G., Liu, H., & Lu, H. (2016). Glucagon-like peptide-1(GLP-1) receptor agonists: Potential to reduce fracture risk in diabetic patients? British Journal of Clinical Pharmacology, 81(1), 78–88. https://doi.org/10.1111/bcp.12777
Maršić, N., Mikulič-Petkovšek, M., & Štampar, F. (2014). Grafting influences the phenolic profile and carpometric traits of fruits of greenhouse-grown eggplant (Solanum melongena L.). Journal of Agricultural and Food Chemistry, 62, 10504–10514. https://doi.org/10.1021/jf503338m
Medina‑Medrano, J., Almaraz‑Abarca, N., González‑Elizondo, M., Uribe‑Soto, J., González‑Valdez, L., & Herrera‑Arrieta, Y. (2015). Phenolic constituents and antioxidant properties of five wild species of Physalis (Solanaceae). Botanical Studies, 56(24), 1–13. https://doi.org/10.1186/s40529-015-0101-y
Ministry of Health, Republic of Indonesia. (2020). Decree of the minister of health of the Republic of Indonesia number KK.01.07/Menkes/603/2020 concerning national guidelines for medical services for the management of type 2 diabetes mellitus for adults. https://yankes.kemkes.go.id/unduhan/fileunduhan_1610340996_61925.pdf
Nguyen, K., Nguyen, N., & Kim, K. (2021). Determination of phenolic acids and flavonoids in leaves, calyces, and fruits of Physalis angulata L. in Viet Nam. Pharmacia, 68(2), 501–509. https://doi.org/10.3897/PHARMACIA.68.E66044
Niño-Medina, G., Urías-Orona, V., Muy-Rangel, M., & Heredia, J. (2017). Structure and content of phenolics in eggplant (Solanum melongena) - A review. South African Journal of Botany, 111, 161–169. https://doi.org/10.1016/j.sajb.2017.03.016
Nisa, P., Adzani, A., Amalia, S., Maulidian, R., Yuniar, E., Mufidah, F., & Fajrin, F. (2022). Theobroma cacao L. (cocoa) pod husk as a new therapy for transient receptor protein vanilloid-1 (TRPV1)-targeted diabetic neuropathy: An in-silico study. Pharmacy Education, 22(2), 104–108. https://doi.org/10.46542/pe.2022.222.104108
Nwanna, E. (2013). Inhibitory effects of methanolic extracts of two eggplant species from South-western Nigeria on starch hydrolysing enzymes linked to type-2 diabetes. African Journal of Pharmacy and Pharmacology, 7(23), 1575–1584. https://doi.org/10.5897/ajpp2013.3606
Pan, J., Zhang, Q., Zhang, C., Yang, W., Liu, H., Lv, Z., Liu, J., & Jiao, Z. (2022). Inhibition of dipeptidyl peptidase-4 by flavonoids: Structure–activity relationship, kinetics and interaction mechanism. Frontiers in Nutrition, 9, 1–17. https://doi.org/10.3389/fnut.2022.892426
Proencą, C., Freitas, M., Ribeiro, D., Tomé, S., Araújo, A., Silva, A., Fernandes, P., & Fernandes, E. (2019). The dipeptidyl peptidase-4 inhibitory effect of flavonoids is hindered in protein rich environments. Food and Function, 10(9), 5718–5731. https://doi.org/10.1039/c9fo00722a
Rahmayanti, F., Pratoko, D., & Fajrin, F. (2022). The binding prediction of 6-paradol and its derivatives on TRPV1 agonist as a new compound for treating painful diabetic neuropathy. Jurnal Ilmu Dasar, 23(2), 127. https://doi.org/10.19184/jid.v23i2.23030
Raju, P., & Mamidala, E. (2015). Anti-diabetic activity of compound isolated from Physalis angulata fruit extracts in alloxan induced diabetic rats. The American Journal of Science and Medical Research, 1(1), 40–43. https://doi.org/10.17812/ajsmr2015111
Riyanti, S., Suganda, A., & Sukandar, E. (2016). Dipeptidyl peptidase-IV inhibitory activity of some Indonesian medicinal plants. Asian Journal of Pharmaceutical and Clinical Research, 9(2), 375–377. https://journals.innovareacademics.in
Saidu, Y., Muhammad, S., Abbas, A., Onu, A., Tsado, I., & Muhammad, L. (2017). In vitro screening for protein tyrosine phosphatase 1B and dipeptidyl peptidase IV inhibitors from selected Nigerian medicinal plants. Journal of Intercultural Ethnopharmacology, 6(2), 154–157. https://doi.org/10.5455/jice.20161219011346
Shaikh, S., Lee, E., Ahmad, K., Ahmad, S., Lim, H., & Choi, I. (2021). A comprehensive review and perspective on natural sources as dipeptidyl peptidase-4 inhibitors for the management of diabetes. Pharmaceuticals, 14(6). https://doi.org/10.3390/ph14060591
Sigma Aldrich. (2014). Product information DPP4 activity assay kit. https://www.sigmaaldrich.cn/
Singh, A., Yadav, D., Sharma, N., & Jin, J. (2021). Dipeptidyl peptidase (DPP)‐iv inhibitors with antioxidant potential isolated from natural sources: A novel approach for the management of diabetes. Pharmaceuticals, 14(6). https://doi.org/10.3390/ph14060586
Singla, R., Kumar, R., Khan, S., Mohit, Kumari, K., & Garg, A. (2019). Natural products: Potential source of DPP-IV Inhibitors. Current Protein & Peptide Science, 20(12), 1218–1225. https://doi.org/10.2174/1389203720666190502154129
Takahama, U., & Hirota, S. (2018). Interactions of flavonoids with α-amylase and starch slow down its digestion. Food and Function, 9(2), 677–687. https://doi.org/10.1039/c7fo01539a
Tuersuntuoheti, T., Pan, F., Zhang, M., Wang, Z., Han, J., Sun, Z., & Song, W. (2022). Prediction of DPP-IV inhibitory potentials of polyphenols existed in Qingke barley fresh noodles: in vitro and in silico analyses. Journal of Food Processing and Preservation, 46(10). https://doi.org/10.1111/jfpp.16808
WHO. (2022). Diabetes. https://www.who.int/news-room/fact-sheets/detail/diabetes
Wu, T., Luo, J., & Xu, B. (2015). In vitro antidiabetic effects of selected fruits and vegetables against glycosidase and aldose reductase. Food Science and Nutrition, 3(6), 495–505. https://doi.org/10.1002/fsn3.243
Wu, X., Zhang, S., Liu, X., Shang, J., Zhang, A., Zhu, Z., & Zha, D. (2020). Chalcone synthase (CHS) family members analysis from eggplant (Solanum melongena L.) in the flavonoid biosynthetic pathway and expression patterns in response to heat stress. PLoS ONE, 15(4), 1–18. https://doi.org/10.1371/journal.pone.0226537
Yaribeygi, H., Atkin, S., & Sahebkar, A. (2019). Natural compounds with DPP-4 inhibitory effects: Implications for the treatment of diabetes. Journal of Cellular Biochemistry, 120(7), 10909–10913. https://doi.org/10.1002/jcb.28467
