Anti-inflammatory activity of Eucheuma denticulatum from Warambadi coast: In-vivo study model of carrageenan-induced paw oedema
DOI:
https://doi.org/10.46542/pe.2023.232.216222Keywords:
Anti-inflammatory, Antioxidant, Carrageenan, Eucheuma denticulatum, Paw oedemaAbstract
Background: Chronic inflammatory diseases have been declared the leading cause of mortality in the world, with more than 50% of deaths associated with inflammatory cases. Based on previous studies, red algae contained antioxidants such as phenolics and flavonoids, which are correlated to their anti-inflammatory activity, including Eucheuma denticulatum.
Objective: This study aims to analyse antioxidants and anti-inflammatory activity as well as the phenolic and flavonoid content of red algae E. denticulatum ethyl acetate extract (EDEE).
Method: EDEE was macerated in ethyl acetate and the antioxidant activity was performed using the (2,2-diphenyl-1-picryl-hydrazyl-hydrate) DPPH method. Total phenolic and flavonoid content was quantified using the Folin-Ciocalteu and AlCl3 methods. The anti-inflammatory activity was analysed by measuring the percentage of anti-inflammatory activity in carrageenan-induced mice paw edema.
Result: The results showed that the Inhibition concentration 50 (IC50) of EDEE was 1031.5 ppm with a total phenolic and flavonoid content of 81.34 ± 0.996mg gallic acid equivalents (GAE/g) and 5.64 ± 0.12 mg quercetin equivalents (QE/g) extract, respectively. In vivo study showed that EDEE of 20, 50, and 100mg/kg body weight (BW) provided the percentage of anti-inflammatory activity of 53.49%, 86.63%, and 78.49% respectively, which is significantly different (p<0.05) compared to the negative control.
Conclusion: EDEE 50mg/kg BW had the highest anti-inflammatory activity due to the presence of phenolic compounds.
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