Formulation and in vitro study of nanoparticles loaded Anredera cordifolia leaf extract as anti-acne
DOI:
https://doi.org/10.46542/pe.2023.232.6570Keywords:
Anti-acne, Antibacterial inhibition test, Chitosan, Nanoparticles, Sodium tripolyphosphateAbstract
Background: The ethanolic extract of Anredera cordifolia (AC) leaves has previously been investigated to have antibacterial activity against Propionibacterium acnes. An active antibacterial compound against propionibacterium acnes was flavonoid.
Objective: This study aimed to compare antimicrobial activity differences between nanoparticles and extract alone.
Method: AC leaves were extracted and then formulated into nanoparticles using chitosan as the polymer and sodium tripolyphosphate (Na-TPP) as a crosslinker. The nanoparticle-preparation method was ionic gelation, while the antibacterial study used the disc method.
Result: Nanoparticle evaluations showed an average size of 84.93±15.36nm, a polydispersity index of 0.22, a percent entrapment efficiency of 55.06±1.05%, and an average yield of 11.00±1.27%. Based on statistical t-test analysis, nanoparticles differed significantly from extract alone in antimicrobial activity.
Conclusion: Nanoparticles significantly increase the antibacterial effectiveness of extract through the synergism effect between chitosan and flavonoid content in the extract. Hence nanoparticles serve good potency to deliver extract for acne therapy.
References
Alba, M.T, Pelegrin, G.C.M., & Sobottka, A.M. (2020). Pharmacognosy Ethnobotany, Ecology, Pharmacology, And Chemistry of Anredera Cordifolia (Basellaceae), Pharmacognosy, 71, 1-11. https://doi.org/10.1590/2175-7860202071060
Basyuni, M., Ginting, P.Y.A., & Lesmana, I. (2017). Phytochemical analysis of Binahong (Anredera Cordifolia) leaves extract to inhibit In Vitro growth of Aeromonas Hydrophila. AIP Conference Proceedings 1904. 020072. https://doi.org/10.1063/1.5011929
Darmawati. (2015). Isolasi dan identifikasi senyawa golongan flavonoid pada daun nangka (Artocarpusheterophyllus lmk) dan aktivitas antibakteri terhadap bakteri Staphylococcus aureus. Jurnal Kimia, 9(2), 203-210. https://doi.org/10.24843/JCHEM.2015.v09.i02.p10
Emeje,. M.O., Obidike, I.C., Akpabio, E.I., & Ofoefule, S.I. (2012). Nanotechnology in drug delivery. In A.D. Sezer (Eds.), Recent Advances in Novel Drug Carrier Systems(pp.69-106). IntechOpen. https://doi.org/10.5772/51384
Hasbullah, A.H.A. (2016). Kandungan senyawa saponin pada daun, batang dan umbi tanaman Binahong [Saponin compound content on the leaves, stems and tubers of Anredera cordifolia (Ten) Steenis. PLANTROPICA: Journal of Agricultural Science, 4(1), 20-2.7
Helmidanora, R., Sukawaty, Y., & Warnida, H. (2020). Penetapan kadar flavonoid daun binahong (anredera cordifolia (ten) steenis) dengan spektrofotometri uv-vis. Scientia Jurnal Farmasi dan Kesehatan, 10(2), 192-199. http://dx.doi.org/10.36434/scientia.v10i2.230
Kemenkes, R.I. (2017). Indonesian Herbal Pharmacopeia 2nd edition. Kementrian Kesehatan RI. Jakarta. 561
Krisanti, E., Wahyunisa, M.P., Hijrianti, N., & Mulia, K. (2019). Chitosan-alginate matrices loaded with leaf extracts of Anredera cordifolia Steenis as a gastrointestinal extended-release formulation. AIP Conference Proceedings, 2193(1):030001. https://doi.org/10.1063/1.5139338
Lazarido, M., Christodoulou, E., Nerantzaki, M., Kostoglou, M., Lambropoulou, D.A., Katsarou, A., Pantopoulos, K., & Bikiaris, D.N. (2020). Characterization of Chitosan-Nanoparticles Loaded with the Iron Chelator Deferoxamine Mesylate (DFO). Pharmaceutics, 12(3), 1-27. https://doi.org/10.3390/pharmaceutics12030238
Mardliyati, E.S., El Muttaqien, & Setyawati D.R. (2012). Sintesis nanopartikel kitosan- tripoli phosphate dengan metode gelasi ionik : pengaruh konsentrasi dan rasio volume terhadap karakteristik partikel. Prosiding Pertemuan Ilmiah Ilmu Pengetahuan Dan Teknologi Bahan, 90–93. https://digilib.batan.go.id/ppin/katalog/file/1411-2213-2012-1-0901.pdf
Yuda, P.E.S.K, Cahyaningsi, E., & Winariyanthi, N.LP.Y. (2017). Phytochemical Screening and Layer Chromatography Analysis of Patikan Kebo Plant Extracts (Euphorbia hirta L). Medicamento, 3(2), 61-70. https://www.neliti.com/publications/329088/skrining-fitokimia-dan-analisis-kromatografi-lapis-tipis-ekstrak-tanaman-patikan#id-section-content
Nagy, A., Harrison, A., Sabbani, S., Munson R. S., Dutta P. K., & Waldman, W. J. (2011). Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action. International Journal of Nanomedicine, 6, 1833. https://doi.org/10.2147/ijn.s24019
Parwati, N.K.F., Napitulu M., & Diah, A.W.M. (2014). Antioxidant Activity Test of Binahong Leaf Extract (Anredera cordifolia (Tenore) Steen) with 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) Using a UV-Vis Spectrophotometer. Jurnal Akademika Kimia, 3(4), 206-213. http://jurnal.untad.ac.id/jurnal/index.php/JAK/article/view/7836
Pelen, S.A., Wullur, & Citraningtyas, G. (2016). Formulasi sediaan gel antijerawat minyak atsiri kulit batang kayu manis (Cinnamomum burmanii) dan uji aktivitas terhadap bakteri Staphylococcus aureus. Pharmacon, 5(4), 136–144. https://doi.org/10.35799/pha.5.2016.13984
Rachmawati, S. (2008). Studi Makroskopik dan Skrining Fitokimia Daun Anredera cordifolia (Ten) Steenis [Macroscopic Studies and Leaf Phytochemical Screening of Anredera cordifolia (Ten) Steenis. Skripsi. Universitas Airlangga. Available at: https://repository.unair.ac.id/10519/
Utami, E.R. (2012). Antibiotika, resistensi, dan rasionalitas terapi. El-Hayah Jurnal Biologi, 1(4), 124–138. https://doi.org/10.18860/elha.v1i4.1783
Verma, S., Utreja, & P., Kumar, L. (2018). Nanotechnological Carriers for Treatment of Acne. Recent Patents on Ati-Infective Drug Discovery, 13(2), 105-126. https://doi.org/10.2174/1574891x13666180918114349
Worldwide, M.I. (2011). Dynamic Light Scattering, Common Terms Defined. Malvern Instruments Limited. Malvern, UK, pp. 1–6. Available at: https://facilities.bioc.cam.ac.uk/files/media/dls_terms_defined_malvern.pdf
Xie, Y., Yang, W., Tang, F., Chen, X, & Ren, L. (2015). Antibacterial activities of flavonoids: structure-activity relationship and mechanism. Current Medicinal Chemistry, 22(1), 132-49. https://doi.org/10.2174/0929867321666140916113443
