Hand sanitiser activity test of eucalyptus (Eucalyptus globulus) oil extract against Bacillus subtilis and Enterococcus faecalis bacteria
DOI:
https://doi.org/10.46542/pe.2023.232.179183Keywords:
Association of official analytical chemists method, Bacillus subtilis, Enterococcus faecalis, Eucalyptus oil, Hand sanitiserAbstract
Background: Bacillus subtillis bacteria can cause meningitis, endocarditis, and eye infections, while Enterococcus can cause meningitis in neonates and endocarditis in adults. Therefore, it is important to use hand sanitiser to avoid bacterial infections. Eucalyptus is an essential oil-producing plant that can inhibit growth and kill bacteria with its compounds, namely linalool and pinocarveol.
Purpose: To make a good eucalyptus oil hand sanitiser and test its effectiveness against Bacillus subtilis and Enterococcus faecalis bacteria.
Method: Making transparent hand sanitiser with a mixture of eucalyptus oil extract. Physical, chemical and antibacterial tests on Bacillus subtilis and Enterococcus faecalis.
Results: The hand sanitiser preparation obtained an acidity of 7, with a specific gravity of 0.8512 g/ml, and a viscosity of 4.14 cP and the cleaning power of Bacillus subtilis and Enterococcus faecalis was 99.9%.
Conclusion: Eucalyptus oil extract hand sanitiser is of good quality and effectively kills Bacillus subtilis and Enterococcus faecalis bacteria.
References
AOAC International Official Methods of Analysis. 2013. Germicidal and Detergent Sanitizing Action of Desinfectants.18th AOAC Official Method 960.09. Revised First Action 2013
Asri Widyasanti, C. L. (2016). Pembuatan Sabun Padat Transparan Menggunakna Minyak Kelapa Sawit (Palm Oil) Dengan Penambahan Bahan Aktif Ekstrak Teh Putih (Camellia sinesis). Jurnal Teknik Pertanian Lampung, 5(3), 125-136
Astiani, D. P., Jayuska, A., & Arreneuz, S. (2014). Uji aktivitas antibakteri minyak atsiri Eucalyptus pellita terhadap bakteri Escherichia coli dan Staphylococcus aureus. Jurnal Kimia Khatulistiwa, 3(3)
Baumgartner, J. C. (2004). Microbiologic aspects of endodontic infections. Journal of the California Dental Association, 32(6), 459-468
Ferreira, C. M., Rosa, O. P. D. S., Torres, S. A., Ferreira, F. B. D. A., & Bernardinelli, N. (2002). The activity of endodontic antibacterial agents against selected anaerobic bacteria. Brazilian Dental Journal, 13, 118-122. https://doi.org/10.1590/S0103-64402002000200008
Ghalem, B.R, and Mohamed, B., 2008, Antibacterial Activity Of Leaf Essential Oils Of Eucalyptus Globulusand eucalyptus Camaldulensis, Afr. J. Pharm. Pharmacol, 2(10), 211-215. https://doi.org/10.5897/AJPP.9000039
Kampf, G., & Ostermeyer, C. (2004). Efficacy of alcohol-based gels compared with simple hand wash and hygienic hand disinfection. Journal of Hospital Infection, 56(SUPPL.2), 13–15. https://doi.org/10.1016/j.jhin.2003.12.026
Kementerian Kesehatan Republik Indonesia. (2017). Pedoman Manajerial Infeksi di Rumah Fasilitas Pelayanan Kesehatan Lainnya
Kerlinger, F. N. (1973). Content analysis. Foundations of Behavioral Research. 2nd ed. (Holt, Rinehart & Winston, Inc., 19)
Kishen, A., George, S., & Kumar, R. (2006). Enterococcus faecalis‐mediated biomineralized biofilm formation on root canal dentine in vitro. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials, and The Australian Society for Biomaterials and the Korean Society for Biomaterials, 77(2), 406-415. https://doi.org/10.1002/jbm.a.30622
Mahary, A., & AGS, D. A. (2021). Pelatihan Pembuatan Hand Sanitizer Sesuai Dengan Standar Who Pada Ibu-Ibu Perwiritan Kab. Batubara. Selaparang Jurnal Pengabdian Masyarakat Berkemajuan, 4(3), 879-882. https://doi.org/10.31764/jpmb.v4i3.5400
Mariana, M.-A., Maricruz, O.-A., Marta, L.-S., Ivonne, P.-X., Ada, M. R.-C., & Sandra, L. C.-H. (2017). Antibacterial activity of extracts of Stevia rebaudiana Bertoni against Staphylococcus aureus, Staphylococcus epidermidis, and Pseudomonas aeruginosa. Journal of Medicinal Plants Research, 11(25), 414–418. https://doi.org/10.5897/jmpr2017.6373
Martin, A., J. Swarbrick, dan A. Cammarata. 1993. Farmasi Fisik: Dasar-dasar Farmasi Fisik dalam Ilmu Farmasetik. Edisi Ketiga. Penerjemah: Yoshita. Jakarta: UI-Press. Hal. 1176-1182
Nopiyanti, H. T., & Agustriani, F. (2016). Skrining Nypa fruticans sebagai Antibakteri Bacillus subtilis, Escherichia coli dan Staphylococcus aureus. Maspari Journal: Marine Science Research, 8(2), 83-90
Nurdianti, L. (2020). Uji Aktivitas Antibakteri Sediaan Obat Kumur Ekstrak Etanol Daun Mangga Harumanis (Mangifera Indica, L) Terhadap Streptococcus Mutans Penyebab Karies Gigi. Journal of Pharmacopolium, 3(1). https://doi.org/10.46772/jophus.v3i01.518
Pratiwi, S. T. (2008). Mikrobiologi farmasi. Erlangga.
Rini, E. P., & Nugraheni, E. R. (2018). Uji Daya Hambat Berbagai Merek Hand Sanitizer Gel Terhadap Pertumbuhan Bakteri Escherichia coli dan Staphylococcus aureus. JPSCR : Journal of Pharmaceutical Science and Clinical Research, 3(1), 18. https://doi.org/10.20961/jpscr.v3i1.15380
Sawitri, D. (2010). Pengaruh Pelatihan Berpikir Positif Pada Efikasi Diri Akademik Mahasiswa (Studi Eksperimen Pada Mahasiswa Fakultas Psikologi Undip Semarang. Pengaruh Pelatihan Berpikir Positif Pada Efikasi Diri Akademik Mahasiswa (Studi Eksperimen Pada Mahasiswa Fakultas Psikologi Undip Semarang, 8(2), 135–144. https://doi.org/10.14710/jpu.8.2.135-144
Soraya, C., Sunnati, S., & Maulina, V. (2016). Efek Antibakteri Ekstrak Batang Serai (Cymbopogoncitratus) Terhadap Pertumbuhan Enterococcus Faecalis. Cakradonya Dental Journal, 8(2)
World Health Organisation. (2009). WHO Guidelines on Hand Hygiene in Health Care First Global Patient Safety Challenge Clean Care is Safer Care. Geneva, Switzerland
