Antioxidant activities of medang lendir (Litsea glutinosa) stem bark
DOI:
https://doi.org/10.46542/pe.2023.232.122125Keywords:
Antioxidant activity, Litsea glutinosa, Total phenolic contentAbstract
Background: Medang lendir (Litsea glutinosa), a member of the Lauraceae family, is typically found in the Kampar Regency area of Riau Province, Indonesia. This plant is frequently used in traditional medicine to treat rheumatism, diarrhea, and dysentery.
Objective: To evaluate the antioxidant activities of L. glutinosa stem bark extracts.
Method: L. glutinosa stem bark was macerated in methanol to obtain methanol extract, followed by partitioning with ethyl acetate to obtain ethyl acetate extract. The antioxidant activities of these extracts were determined using the NO radical scavenging and the DPPH radical scavenging methods. The Folin-Ciocalteu method was also used in this study to determine total phenolic content (TPC).
Result: The antioxidant activities of ethyl acetate and methanol extracts of L. glutinosa stem bark against NO radicals resulted in a small percentage of inhibition (%I < 0). Meanwhile, for DPPH radical scavenging, the antioxidant activity of the ethyl acetate extract (IC50 = 222.03 ug/mL) was greater than the methanol extract (IC50 = 232.81 ug/mL), and the TPC value of the ethyl acetate extract (23.88 mgGAE/g) was greater than the methanol extract (23.16 mgGAE/g).
Conclusion: These results show that the antioxidant activity based on scavenging DPPH radicals for both extracts was active; however, it was inactive on NO radicals. The ethyl acetate extract possessed a higher TPC value compared to the methanol extract.
References
Almurdani, M., Zamri, A., Nugroho, T.T., Karim, J., Eryanti, Y., Hendra, R. & Teruna, H.Y. (2020). Antioxidant and Antidiabetic Activities of Mempening (Lithocarpus bancanus) Leaves. Pharmacognosy Journal. 12(2):328-334. https://doi.org/10.5530/pj.2020.12.52
Aryal, S., Baniya, M.K., Danekhu, K., Kunwar, P., Gurung, R. & Koirala, N. (2019). Total Phenolic Content, Flavonoid Content and Antioxidant Potential of Wild Vegetables from Western Nepal. Plants. 8(4):96-108. https://doi.org/10.3390/plants8040096
Dobrinas, S., Soceanu, A., Popescu, V., Carazeanu Popovici, I. & Jitariu, D. (2021). Relationship between Total Phenolic Content, Antioxidant capacity, Fe and Cu Content from Tea Plant Samples at Different Brewing Times. Processes. 9(8):1311-1322. https://doi.org/10.3390/pr9081311
Johari, M.A. & Khong, H.Y. (2019). Total Phenolic Content and Antioxidant and Antibacterial Activities of Pereskia bleo. Advances in Pharmacological Sciences. 2019(Special Issue). https://doi.org/10.1155/2019/7428593
Kamle, M., Mahato, D.K., Lee, K.E., Bajpai, V.K., Gajurel, P.R., Gu, K.S. & Kumar, P. (2019). Ethnopharmacological Properties and Medicinal Uses of Litsea cubeba. Plants. 8 (6):150-153. https://doi.org/10.3390/plants8060150
Lestari, Y.S., Yuanmizesfi, E., Zamri, A. & Teruna, H.Y. (2021). An Antimalarial Constituent from Alkaloid of Medang Lendir (Litsea glutinosa (Lour.) C.B. Rob), AIP Conference Proceeding. 2360(050030):1-4. https://doi.org/10.1063/5.0059599
Mfotie-Njoya, E., Munvera, A. M., Mkounga, P., Nkengfack, A. E. & McGaw, L. J. (2017). Phytochemical Analysis with Free Radical Scavenging, Nitric Oxide Inhibition and Antiproliferative Activity of Sarcocephalus pobeguinii Extracts. BMC Complementary and Alternative Medicine. 7(199):1-9. https://doi.org/10.1186/s12906-017-1712-5
Muflihah, Y. M., Gollavelli, G. & Ling, Y. C. (2021). Correlation Study of Antioxidant Activity with Phenolic and Flavonoid Compounds in 12 Indonesian Indigenous Herbs. Antioxidants. 10(10):1530-1545. https://doi.org/10.3390/antiox10101530
Pradeepa, K., Krishna, V., Venkatesh, Girish Kumar, K., Thirumalesh, B. V. & Naveen Kumar, K. J. (2011). Antibacterial screening of the stem bark and leaf extracts of Litsea glutinosa (Lour.) C.B. Rob - An Ethnomedicinally Important Tree of the Western Ghats. Pharmacognosy Journal. 3(21):72-76. https://doi.org/10.5530/pj.2011.21.13
Pradeepa, K., Krishna, V., Venkatesh, Santosh Kumar, S.R. & Girish Kumar, K. (2013). Antinociceptive Property of Leaves extract of Litsea glutinosa. Asian Journal of Pharmaceutical and Clinical Research. 6(1):182-184. https://innovareacademics.in/journal/ajpcr/Vol6Suppl1/1582.pdf
Sies, H. (1997). Oxidative stress: oxidants and antioxidants. Experimental physiology, 82(2):291–295. https://doi.org/10.1113/expphysiol.1997.sp004024
Sukweenadhi, J., Yunita, O., Setiawan, F., Kartini, Siagian, M.T., Danduru, A.P. & Avanti, C. (2020). Antioxidant Activity Screening of Seven Indonesian Herbal Extract. Biodiversitas. 21(5):2062-2067. https://doi.org/10.13057/biodiv/d210532
Uttara, B., Singh, A. V., Zamboni, P. & Mahajan, R. T. (2009). Oxidative Stress and Neurodegenerative Diseases: a Review of Upstream and Downstream Antioxidant Therapeutic Options. Current neuropharmacology, 7(1):65–74. https://doi.org/10.2174/157015909787602823